Role: Research Engineer

Location: CBRS 2nd floor – University side – office 205

Phone number : 05.19.56.42.33

Email address: emilie.pinault@unilim.fr

 

Research

Research Engineer shared between the U1248 Pharmacology and Transplantation laboratory and the BISCEm platform 

Within U1248, participation in research projects requiring expertise in biochemistry and/or mass spectrometry:

  • Development of sample preparation methods and analytical methods in mass spectrometry necessary for the advancement of research projects (short-chain fatty acids, drugs, etc.)
  • Development of ELISA and Western Blot assays

Within the BISCEm platform, as manager of the mass spectrometry unit:

  • Mass spectrometry services for small and large molecules: development and execution of experiments (sample preparation, LC-MS/MS analyses)
  • Data analysis and presentation of results
  • Cost management (cost calculation, preparation of quotes) and technical resources management (equipment investment decisions, maintenance, and servicing)
  • Supervision and demonstration

Teaching

  • Biotechnology course (UE) of the Master 2 “Bio-Health”, Limoges: presentation and tour of the mass spectrometry platform
  • Jury member for the BTS “BioAnalyses et Contrôles”, Lycée R. Dautry, Limoges

Career path

2020-present U1248 Pharmacology and Transplantation – Inserm, University of Limoges
2018-present BISCEm Platform – US042 Inserm UAR2015 CNRS, University of Limoges, Limoges University Hospital
2006-2018 Proteomics Platform, then Shared Service for Research and Biomolecule Analysis of Limoges (SCRABL)
2006-2020 Animal Molecular Genetics Unit – UMR 1061 INRA, then PEREINE Laboratory, University of Limoges
2006 Engineering degree in Biochemical and Food Engineering, Microbial Genetics, with a specialization in Post-Genomic Technology – National Institute of Applied Sciences (INSA), Toulouse (31)
Master 2 Research: Microbiology and Industrial Catalysis – INSA Toulouse (31)
2001 Scientific Baccalaureate, specialization in Physics and Chemistry – Lycée Rabelais, Chinon (37)

 

Skills

Biological Sample Preparation

  • Extraction of small molecules from cells or biological fluids: liquid-liquid extraction, solid-phase extraction (SPE)
  • Protein extraction from cell cultures, biological fluids (blood, urine, etc.), animal/human tissues (muscle, liver, etc.), or plant tissues (wood, seedlings, etc.)
  • Protein purification/concentration methods by precipitation, depletion of major proteins from human samples, or enrichment using the ProteoMiner system

Separation Techniques

  • Electrophoresis under denaturing and non-denaturing conditions, isoelectric focusing and two-dimensional gels, off-gel isoelectric focusing
  • Protein transfer in semi-liquid medium (Western Blot) and immunodetection

Mass Spectrometry Analysis

  • Liquid chromatography systems coupled to mass spectrometers: LCMS8060 (Shimadzu) and TimsTOF Pro2 (Bruker) coupled with LC40 Nexera X2 (Shimadzu) or nanoElute 2 (Bruker)
  • Software for system control and data acquisition: LabSolutions (Shimadzu), TimsControl / Hystar (Bruker), Analyst (Sciex)
  • Data processing software for identification (ProteinPilot, Mascot, ProteoScape) and quantification (Swath 2.0, MultiQuant, LabSolutions Insight, MetaboScape)