The CRIBL laboratory is proud to announce the publication of the book RNA Analysis: Methods and Protocols, published by Springer Nature, to which Laurent Delpy, a researcher within our unit, contributed as an editor. This book, part of the renowned Methods in Molecular Biology series, offers an updated overview of the latest technologies for RNA analysis.

Innovative protocols to explore RNA biology

Designed as a practical guide, this volume covers both fundamental methods and innovative approaches that enhance the understanding of RNA biology. The different chapters—written by international experts in the field and organized into four main parts—describe detailed protocols for the isolation, amplification, detection, modification, and analysis of RNA. The book also provides numerous tips for successfully performing these delicate experiments while avoiding common pitfalls.

A broadly engaged CRIBL team

The book RNA Analysis: Methods and Protocols highlights the strong involvement of the CRIBL laboratory in this cutting-edge research area. Several researchers from CRIBL are among the authors of this reference work. Their collective participation reflects the wealth of expertise gathered within our laboratory, as well as the team’s commitment to the development and dissemination of innovative methods for RNA study.

International scientific influence

Through their contributions on original protocols and advanced methodological approaches, these authors demonstrate CRIBL’s ability to play a leading role in the international scientific community by sharing know-how and technical advances with specialists worldwide. This collaboration also underscores the dynamic team spirit within the laboratory and the central position it holds in RNA biology research at both national and international levels.

A propos de ce livre

This volume explores the latest technologies for RNA analysis, covering fundamental techniques and innovative approaches that expand our understanding of RNA biology. The chapters in this book are organized into four parts: reverse transcription and RNA capture methods; high-throughput sequencing methods; RNA maturation: splicing, polyadenylation, and epitranscriptomic modifications; and RNA surveillance: nonsense-mediated mRNA decay. These chapters aim to provide detailed protocols for select techniques used by molecular and computational biologists to isolate, amplify, detect, modify, and analyze RNA to gain insight into a wide range of cellular functions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, RNA Analysis: Methods and Protocols is a valuable resource for students, technicians, and senior scientists who want to learn more about this dynamic field of RNA research.

Keywords

RNA-sequencing methods | N6-methyladenosine | Northern Blot | CLIP-seq | NMD inhibitors