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Analysis and characterization of antiviral drug-resistant cytomegalovirus isolates from solid organ transplant recipients.
- Pubmed_id: 12198609
- Journal: The Journal of infectious diseases
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Genbank_id: No known Genbank_id
- Date: Sep 2002
- Volume: 186
- Issue: 6
- Pages: 760-8
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Author(s):
Lurain NS,
Bhorade SM,
Pursell KJ,
Avery RK,
Yeldandi VV,
Isada CM,
Robert ES,
Kohn DJ,
Arens MQ,
Garrity ER,
Taege AJ,
Mullen MG,
Todd KM,
Bremer JW,
Yen-Lieberman B,
- Abstract: The development of cytomegalovirus (CMV) disease and subsequent emergence of drug-resistant strains was examined in a large group of solid organ transplant recipients; drug-resistant CMV was detected in a total of 30 transplant recipients (20 lung, 5 kidney, 4 heart, and 1 liver). Drug resistance was confirmed both phenotypically and genotypically. The sequences of drug-resistant CMV strains from the same patient differed from drug-susceptible baseline sequences only at single sites previously confirmed to confer drug resistance. At least 1 isolate from each patient had a mutation in the UL97 phosphotransferase coding sequence. Mutations in the DNA polymerase gene were found in 6 of 38 sequenced strains. Lung transplant recipients had the highest incidence of drug-resistant virus: of the 30 patients, 28 were CMV-seronegative transplant recipients of CMV-seropositive organs, which strongly supports the premise that drug resistance is most prevalent in that transplant population.
