Codex_V5

Growth and drug resistance phenotypes resulting from cytomegalovirus DNA polymerase region III mutations observed in clinical specimens.

Abstract: Recombinant phenotyping of cytomegalovirus (CMV) pol region III mutations from clinical specimens showed that T813S and G841A each conferred foscarnet resistance and approximately threefold increased ganciclovir resistance; adding the UL97 mutation C592G increased ganciclovir resistance to approximately sixfold. Bacterial artificial chromosome CMV clones containing pol mutation L845P were nonviable unless repaired with the wild-type sequence.

Mutant	Gene	Virus	Antiviral	EC50 ratio	Phenotype
T813S	UL54	Human betaherpesvirus 5	Ganciclovir	2.50	Low level resistance
T813S	UL54	Human betaherpesvirus 5	Cidofovir	2.90	Low level resistance
T813S	UL54	Human betaherpesvirus 5	Foscarnet	4.90	Intermediate level resistance
G841A	UL54	Human betaherpesvirus 5	Ganciclovir	3.20	Intermediate level resistance
G841A	UL54	Human betaherpesvirus 5	Cidofovir	2.60	Low level resistance
G841A	UL54	Human betaherpesvirus 5	Foscarnet	4.30	Intermediate level resistance