Codex_V5

Rapid detection of human cytomegalovirus UL97 and UL54 mutations for antiviral resistance in clinical specimens.

Pubmed_id: 23668613
Journal: Microbiology and immunology
Genbank_id: No known Genbank_id
Date: May 2013
Volume: 57
Issue: 5
Pages: 396-9
Author(s): Daikoku T, Saito K, Aihara T, Ikeda M, Takahashi Y, Hosoi H, Nishida T, Takemoto M, Shiraki K,

Abstract: Drug-resistant cytomegalovirus appears during prolonged anti-cytomegalovirus therapy. Assays for human cytomegalovirus viral protein kinase (UL97) and viral DNA polymerase (UL54) gene mutations conferring drug resistance have been used rather than susceptibility assays to assess clinical specimens. In this study a sensitive system for genotype assay of UL97 and UL54 in clinical specimens with as few as six copies/µg of DNA was developed.

Mutant	Gene	Virus	Antiviral	EC50 ratio	Phenotype
V787A	UL54	Human betaherpesvirus 5	Ganciclovir	2.50	Low level resistance
V787A	UL54	Human betaherpesvirus 5	Cidofovir
V787A	UL54	Human betaherpesvirus 5	Foscarnet	3.50	Intermediate level resistance
C518Y	UL97	Human betaherpesvirus 5	Ganciclovir	12.00	High level resistance