Codex_V5

Characterization of multiple cytomegalovirus drug resistance mutations detected in a hematopoietic stem cell transplant recipient by recombinant phenotyping.

Abstract: Cytomegalovirus resistance to antivirals is a major problem in transplant recipients. We evaluated the impact of five mutations (A594V, L595F, and E655K in the UL97 gene and V526L and E756K in the UL54 gene), detected in a blood sample from a stem cell transplant recipient, on drug susceptibilities and replicative capacities of recombinant viruses.

Mutant	Gene	Virus	Antiviral	EC50 ratio	Phenotype
V526L	UL54	Human betaherpesvirus 5	Ganciclovir	5.50	High level resistance
V526L	UL54	Human betaherpesvirus 5	Cidofovir	2.50	Low level resistance
V526L	UL54	Human betaherpesvirus 5	Foscarnet	1.80	No resistance
E655K	UL97	Human betaherpesvirus 5	Ganciclovir	1.70	No resistance